Plasmi::db is a project management tool for molecular biology

• Storage of sequence data
• Automation of repetitive tasks, such as translation of open reading frames into protein sequences and molecular weight calculation
• Cross-checking of nucleotide sequences and their properties, such as molecular weights and extinction coefficients of derived protein sequences
• Sample labeling and tracking for downstream applications such as crystallogenesis
• Oligonucleotide 'primer' design tools based on standard cloning strategies.

Therefore, we have produced a tool which stores plasmid DNA sequences, their desired product sequences and helps keep a clear overview of the processes involved in vector construction.

Figure 1. A user can attribute a protein "Target", to a Complex. This later allows the selection of targets according to the complex to which the target belongs. The "complex" page allows a quick overview of the various projects.

Figure 2. When a complex is selected, the protein targets are sorted to that the complex subunits of interest are displayed.

This allows coherent storage of information about:

• Standard cloning or expression plasmids (e.g. their antibiotic selection markers, any RNA polymerase promoters).
  
• Database sequences you may be currently working on (their accession numbers, sequences, coding sequences).
  
• Products you may wish to express or purify from them.  e.g. His-tagged protein or RNA run-off transcripts etc.
• Primers you have ordered.
• Restriction enzymes.
• DNA sequencing data.
• Protein prep yields and data about purity.